Grobet Luc

Researcher, Professor
Researcher publications

 

Research subjects

Current research is carried on in two main areas : embryonic stem cells and transgenesis. Research on embryonic stem cells is aiming at (i) contributing to understand the mechanisms of pluripotency in response to hypoxic culture conditions and (ii) develop an efficient, biologically safe and automatable  method for cryopreserving stem and related cells in such a way that their intended therapeutic use in humans would not be compromised. The unit is actively collaborating with the  platform  of mammalian transgenesis of the FARAH center. In the chicken and in the turkey, the unit focuses on developing efficient methods for deriving, culturing, genetic engineering and cryopreserving primordial germ cells with two goals : (i) development of an efficient transgenesis module by combined use of long genomic insert –based transgenesis and cre-loxP-based site specific recombination, and (ii) improvemnt of current cryopreservation techniques of male and female avian germplasm for genetic resources preservation purposes.

Equipment / technologies

Zygote pronuclear microinjection station (inverted microscope, micromanipulators, camera)

Equipments for manipulating avian embryos

Equipments for eucaryotic cell derivation and culture

Equipments for routine molecular biology and cloning

Academic distinctions

Laureate of the national award of AGIM 2000 (General Association of the Medicinal Industry) bestowed by the Royal Academy of Medicine in Belgium

Double-muscling in mammals patent Nrs: WO9902667, US6103466, US2003129171, AU756620B

Transgenic mouse having conditional knock-out of myostatin function patent Nr: US2004158884

Most representative publications

  • GROBET L., et al. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nat Genet. 1997 Sep, 17(1):71-4
  • GROBET L., et al. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle. Mamm Genome. 1998 Mar, 9(3):210-3 http://orbi.ulg.ac.be/handle/2268/34391 
  • GROBET L., et al . Modulating skeletal muscle mass by postnatal, muscle-specific inactivation of the myostatin gene. Genesis. 2003 Apr, 35(4):227-38 http://orbi.ulg.ac.be/handle/2268/4433 
  • Pirottin D., GROBET L., Adamantidis A., Farnir F., Herens Ch., Daa Schrøder H., Georges M. Transgenic engineering of male-specific muscular hypertrophy. Proc Natl Acad Sci U S A. 2005 May 3, 102(18): 6413–6418. Published online 2005 April 25. doi: 10.1073/pnas.0502426102 http://orbi.ulg.ac.be/handle/2268/94431 
  • Tonus C., Cloquette K., Ectors F., Piret J., Gillet L., Antoine N., Desmecht D., Vanderplasschen A., Waroux O. et GROBET L. Long term-cultured and cryopreserved primordial germ cells from various chicken breeds retain high proliferative potential and gonadal colonisation competency. Reprod Fertil Dev. 2014 Oct 9. doi: 10.1071/RD14194. Epub ahead of print http://orbi.ulg.ac.be/handle/2268/173837 

Information updated by the researcher on 15/10/2015.

PhD students

Tonus Céline

Vanderzwalmen Pierre

Postdoc or other

Connan Delphine